Ever since the term Biotechnology was coined by Karl Early, the Hungarian Engineer (1919), advancements in research and development in biotechnology took rapid pace to benefit human, animal and plant life. With concern to plants and agriculture, emergence of biotechnology applications could be linked broadly to biosystematics, synthetic biology, bioinformatics and nanobiotechnology as frontline areas, and specifically to plant tissue culture and genetic transformation studies. Though innovative research protocols in biotechnology had started around 60 years ago, major breakthrough were reported in first 20 years [1960-1980] in both areas of plant tissue culture and genetic transformation, applications to welfare of human and animal life. It is speculated research will find applications in the medical (clinical) and pharmaceutical, agriculture and environment arenas. The written text on Concepts in Plant Tissue Culture and Genetic Transformation (Theory and Practice) is designed to imparting knowledge and practical know-how to graduate and post-graduate students of agriculture pursuing advance learning in biotechnology with emphasis on plant tissue culture and the fundamentals of gene transfer studies.